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KMID : 0370219980420030336
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Yakhak Hoeji
1998 Volume.42 No. 3 p.336 ~ p.344
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Embryotoxicity of Ochratoxin A in Cultured Rat Embryonic Midbrain Cells and Whole Embryos
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홍진태/Hong JT
박귀례/한순영/박기숙/김형식/오세동/박희정/이이다/장성재/Park KL/Han SY/Park KS/Kim HS/Oh SD/Park HJ/Lee RD/Jang SJ
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Abstract
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Effects of ochratoxin A (OTA) on embryo development were studied in cultured whole embryos from 9.5 day gestation rat for 48 h. OTA (more than 0.5mcg/ml) induced microcephaly in the cultured rat whole embryos. Protein and DNA content, and DNA synthesis were significantly inhibited by OTA. We next examined whether the microcephaly seen in cultured whole embryo partially results from inhibition of differentiation of embryonic midbrain cells. Embryonic midbrain cells were extracted from 12 day gestation rat embryos, and cultured for 96 hr. OTA ibhibited cell differentiation about 50% over control. We also tested whether OTA-induced embryotoxicity would be associated with oxidative damages. We measured the gamma-glutamyltranspeptidase (gamma-GT) and glutathione peroxidase (GPX) activities, and glutathione (GSH) content in both cultured whole embryos and embryonic midbrain cells. OTA decreased GSH content, whereas slightly increased gamma-GT activity, but GPX activity was not significantly changed. These results show that OTA caused the microcephaly and its effect may be partially due to the inhibition of cell differentiation of embryonic midbrain cells, but the role of oxidative damages is not clear in embryotoxicity.
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